Intracytoplasmic delivery of anionic proteins

Anionic regions in nuclear proteins

Recent technological advances have generated a flood of protein sequence data, much of it deduced from the DNA sequences of cloned genes. In this commentary I will discuss an unexpected sequence motif found in a number of proteins that are thought to interact directly or indirectly with DNA. The motif, which I will term an Aregion (acidic region), is a local high concentration of acidic residue...

Oral Delivery of Proteins

Drug delivery technologies have the potential to enable drug candidates with poor pharmaceutical or biopharmaceutical properties, both for macromolecule and traditional compounds. While there have been many success stories to date, the future offers even more promise. In this article, the author surveys the top ten areas in drug delivery looking forward. William J. Lambert, PhD, is the senior v...

Tryptophan-based chromophore in fluorescent proteins can be anionic

Cyan fluorescent proteins (CFP) with tryptophan66-based chromophore are widely used for live cell imaging. In contrast to green and red fluorescent proteins, no charged states of the CFP chromophore have been described. Here, we studied synthetic CFP chromophore and found that its indole group can be deprotonated rather easily (pKa 12.4).We then reproduced this effect in the CFP mCerulean by pl...

Triplet pregnancy and delivery after intracytoplasmic injection of round-headed spermatozoa.

Intracytoplasmic sperm injection (ICSI) of round-headed spermatozoa into mature oocyte resulted in normal fertilization, embryo development and pregnancy in a 28 year old female. The husband had a long history of primary infertility. Three ICSI attempts were carried out and fertilization and embryo development occurred in all trials. However, only the third trial led to a pregnancy, which prove...

Formulation and Delivery of Proteins and Peptides